Comparing Complex Protein Samples Using Two‐Dimensional Polyacrylamide Gels
نویسندگان
چکیده
منابع مشابه
Photopolymerized cross-linked polyacrylamide gels for on-chip protein sizing.
A new method for on-chip sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of proteins is reported. Miniaturization of SDS-PAGE has attracted significant attention because it offers rapid analysis times, excellent resolution, high throughput, and the potential for integration and automation, as compared to conventional counterparts. The presented on-chip SDS-PAGE technique em...
متن کاملPhotopatterned free-standing polyacrylamide gels for microfluidic protein electrophoresis.
Designed for compatibility with slab-gel polyacrylamide gel electrophoresis (PAGE) reagents and instruments, we detail development of free-standing polyacrylamide gel (fsPAG) microstructures supporting electrophoretic performance rivalling that of microfluidic platforms. For the protein electrophoresis study described here, fsPAGE lanes are comprised of a sample reservoir and contiguous separat...
متن کاملPhotopatterned polyacrylamide gels enable efficient microfluidic protein assays
Permission to make digital or hard copies of all or part of this work for personal or classroom use is granted without fee provided that copies are not made or distributed for profit or commercial advantage and that copies bear this notice and the full citation on the first page. To copy otherwise, to republish, to post on servers or to redistribute to lists, requires prior specific permission....
متن کاملProtein purification from polyacrylamide gels by sonication extraction.
A protein purification procedure using sonication extraction from polyacrylamide gels (PAGE), which involves identification of a particular protein band and its excision, homogenization, sonication, and subsequent passage through a Sephadex G-25 minicolumn, is reported. Our results show a high degree of recovery regardless of the nature of the protein (soluble or membrane bound) or the characte...
متن کاملFormation and Structure of Polyacrylamide Gels
Acrylamide and N,N-methylenebis(acry1amide) (AAm-Bis) copolymerization has been investigated in water at a monomer concentration of 1.8 w/v %. Conversion of monomer and pendant vinyl groups was measured as a function of the reaction time up to the onset of macrogelation. Experimental results indicate that 80% of pendant vinyl groups are consumed by cyclization reactions. When the monomer concen...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Current Protocols in Protein Science
سال: 2019
ISSN: 1934-3655,1934-3663
DOI: 10.1002/cpps.87